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By Monique M. Tirion, Daniel ben-Avraham, Kenneth C. Holmes (auth.), James E. Estes, Paul J. Higgins (eds.)

During the interval August 5-9, 1992, and instantly previous the 1992 Gordon study convention on Motile and Contractile structures, the "Third foreign convention at the constitution and serve as of Ubiquitous mobile Protein Actin" used to be held on the Emma Willard tuition in Troy, long island, below the identify "ACTIN '92". This convention occupied with the basic homes and mobile features of actin and actin­ dependent microfilament structures. the 1st convention during this sequence used to be held in 1982, in Sydney, Australia, and hosted by means of Dr. Cristobal G. dos Remedios and Dr. Julian A. Barden, either from the college of Sydney (New South Wales, Austrailia). the second one convention convened in Monza, Italy in June 1987, and used to be equipped by way of Dr. Roberto Colombo, college of Milan (Italy). This 3rd collecting of researchers dedicated to the research of actin and actin-associated proteins was once prepared by means of Dr. James E. Estes, Albany Stratton V A scientific heart and Dr. Paul 1. Higgins, Albany clinical university, who have been assisted via an Organizing Committee which includes Dr. Edward D. Korn (National middle, Lung and Blood Institute, NIH), Dr. Thomas P. Stossel (Massachusetts common Hospital), Dr. Fumio Matsumura (Rutgers University), and Dr. Stephen Farmer (Boston University). This assembly was once devoted to the various pioneering contributions of Professor Fumio Oosawa to the sphere of actin research.

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Additional info for Actin: Biophysics, Biochemistry, and Cell Biology

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Standard molecular marker (m). 31 DISCUSSION Our data show that TNP-A TP can be incorporated into the nucleotide binding site of F-actin by both sonication and polymerisation. By contrast, TNP-A TP failed to incorporate into G-actin. Like ATP, TNP-A TP is hydrolysed during the formation of the actin polymer (dos Remedios and Cooke, 1984). TNP-ADP incorporation into F-actin can be fully and readily reversed by sonication in excess ATP, implying that the affinity of TNP-ADP for F-actin is lower than the native nucleotide.

2) Converting Ca-actin back to Mg-actin is not easy. A very low ratio of [Ca]/[Mg] is necessary, which usually requires the use of Ca-chelators, and a long time (5-10 min) must be allowed for complete exchange. 3) when Ca-actin is polymerized with MgCI 2, even at high MgCl 2 concentration with Ca-chelator simultaneously added, the polymer produced will be significantly contaminated with Ca-actin. 4) Ca-ADP-actin is hard to prepare, since Ca++ binds very weakly to ADPactin and thus denaturation is accelerated.

Bioi. 140:313-320. S. , 1987, Structural aspects of troponin-tropomyosin regulation of skeletal muscle contraction, Ann. Rev. Biophys. Chem. 16:535-559. 34 ACTIN-BOUND NUCLEOTIDE/DIVALENT CATION INTERACTIONS Lewis C. Gershman, Lynn A. Selden, Henry J. Kinosian, and James E. Estes Research and Medical Services Stratton VA Medical Center Albany, New York 12208 and Departments of Medicine and Physiology and Cell Biology Albany Medical College Albany, New York 12208 INTRODUCTION The protein actin is a major constituent of the cytoskeleton of virtually all eukaryotic cells.

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