By Peter J. Verveer
This quantity offers an outline of complicated fluorescence microscopy, protecting a huge variety of tools. each one bankruptcy makes a speciality of a unique procedure and gives a realistic advisor for program in organic platforms. Written within the hugely winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and warding off identified pitfalls.
Authoritative and state of the art, Advanced Fluorescence Microscopy: equipment and Protocols seeks to supply scientists with equipment for organic platforms which are of curiosity.
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Extra resources for Advanced Fluorescence Microscopy: Methods and Protocols
Portera-Cailliau C, Pan DT, Yuste R (2003) Activity-regulated dynamic behavior of early dendritic protrusions: evidence for different types of dendritic filopodia. Grienberger C, Konnerth A (2012) Imaging calcium in neurons. Garaschuk O, Milos R, Konnerth A (2006) Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Golshani P, Portera-Cailliau C (2008) In vivo 2-photon calcium imaging in layer 2/3 of mice. MacLean J, Yuste R (2005) Imaging action potentials with calcium indicators: practical guide.
45). pii: 2140. 3791/2140. PMID: 21113119 46. Schultz BR, Chamberlain JS (2008) Recombinant adeno-associated virus transduction and integration. Holtmaat A, Bonhoeffer T, Chow DK et al (2009) Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Mostany R, Portera-Cailliau C (2008) A craniotomy surgery procedure for chronic brain imaging. J Vis Exp. (12). pii: 680. 3791/680. Cruz-Martin A, Portera-Cailliau C (2010) In vivo imaging of axonal and dendritic structures in developing cortex.
Adenoviruses, lentiviruses, herpes viruses) are increasingly used for gene delivery into neurons, typically via stereotaxic intracranial injections targeted to a particular brain region [44–46]. 2 Cranial Window for In Vivo 2PE Microscopy Two different surgical preparations, glass-covered cranial window and thinned skull, have been developed to get optical access to the brain and image structure and functionality of labeled neurons [47–50]. 3). The thinned skull preparation requires mechanical thinning of the superficial layers of the skull, preserving a thin layer of the bone that allows imaging through it.