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Product inhibition study with maize HMGR. 663 (positively paraboloid) NADPH HS-CoA non-competitive l. ) Inhibition or dissociation constant calculated from the changes in the intercepts on the y-axis in primary plots as a function of inhibitor concentration; Kis: dissociation constant calculated from the change in the slopes. HMGR was purified from microsomes (P 140,000 x g) isolated from 7-day-old etiolated maize seedlings. The enzyme was solubilized by incubation in the presence of Brij W-1 and of glycerol, by a slight modification of the method described for the radish HMGR.

5 0 C; after 3 h the voltage was 700 v, 13 mA, 10 W. Enzyme activity (AACT/HMGS) was assayed as described in the legend to table 4; however, the concentration of Tris buffer was increased by a factor of 4 and 10 roM Fe 2 + and 20 roM EDTA were added. HMGL was assayed as described in Table 4, however at a four-fold concentration of Tris and in the presence of 20 roM MgCI2. 0. 32 THOMAS J. BACH ET AL. '...... '"• 12 'c51 . U ~ '3.. •.. SI y... •..... •.... Y •...... ,... i o ~c lOOCOr--------------------------------------------------------,I.

Mevinolin: Growth Inhibition in Plant Cell Cultures The use of cell cultures permits studies with less of a problem of apoplastic and symplastic transport of the inhibitor to the site of action. For these experiments we used suspension cultures of Silybum marianum l71 ,196 that were grown in the presence of increasing concentrations of mevinolin (0-10 ~) . , was more prominent at the end of the logarithmic growth period (day 6) than at the beginning (day 3 after application of mevinolin). As in intact and excised seedlings of radish, mevinolin gradually affected the accumulation of prenyllipids: sterols> ubiquinone(s) > plastoquinone - carotenoids - chlorophylls.

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