Download Biomembranes Part R by Sidney Fleischer, Becca Fleischer (Eds.) PDF

By Sidney Fleischer, Becca Fleischer (Eds.)

The significantly acclaimed laboratory common, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate at the present time - really a vital e-book for researchers in all fields of existence sciences

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A. 57, 698 (1967). 9 p. V. Viitanen, D. R. Meninck, H. K. Sarkar, W. R. Trumble, and H. R. Kaback, Biochemistry 24, 7628 (1985). io W. R. Lieb, in "Red Cell Membranes: A Methodological Approach" (J. C. Ellory and J. D. ), p. 135. Academic Press, London, 1982. [2] SURVEY OF CARRIER METHODOLOGY 15 concentrations an unspecifically permeant solute may cause an unstoichiometric or nonobligatory countercxchange. Counterexchange or acceleration of uptake by a countersubstrate can also be used to discriminate a carrier transport from a pore-type transport.

24-2s Often a claim is made for the use of only one particular detergent for the isolation of a membrane protein. Therefore, it would seem that no general rules can be applied to the solubilization of membrane proteins. However, this impression is based mainly on an insufficient understanding of the interaction of detergents with the membrane protein and also of the physical chemistry of this process. Also, in some reviews the tolerance of functional membrane proteins for various detergents has not been critically evaluated.

For the generation of antibodies, which are used for screening carrier-specific m R N A and for the subsequent cloning of cDNA. This second use is widely applied, or rather widely attempted, but will succeed only with sufficient specificity of the antibody m R N A screening. 3. Eventually the denatured carrier protein might be reconverted to structural and functional intactness. 4. An entirely different approach m mentioned briefly here--involves immunoprecipitation. Antibodies are first generated against synthetic peptides containing partial sequences of protein, obtained from cDNA-based structure information.

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