By Valerie B. O’Donnell, Ben Maskrey, Graham W. Taylor (auth.), Banafshé Larijani, Rudiger Woscholski, Colin A. Rosser (eds.)
Lipid Signaling Protocols assembles in one quantity a number of the instruments and methodologies wanted by way of the investigator to resolve lipid established signaling and mobilephone functionality. Divided into handy sections, the amount starts through summarizing the actual houses of hydrophobic metabolites in addition to the actual methodologies used for his or her research, which ends up in the second one part and its number of organic equipment, concentrated round the such a lot correct lipids, their corresponding metabolizing enzymes and the popularity proteins. Following the hugely profitable Methods in Molecular Biology™ sequence layout, the chapters supply comfortably reproducible laboratory protocols, lists of priceless fabrics and reagents, and the information on troubleshooting and heading off recognized pitfalls.
Contributed to through most sensible researchers within the box, Lipid Signaling Protocols is a vital source for either skilled and beginner researchers who need a higher knowing of the appliance of actual methodologies within the context of lipid signaling and lipid metabolism in cellphone biology.
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3. Pulfer M, Murphy RC. Electrospray mass spectrometry of phospholipids. Mass Spectrom Rev 2003;22:332–64. 4. Taguchi R, Houjou T, Nakanishi H, Yamazaki T, Ishida M, Imagawa M, Shimizu T. Focused lipidomics by tandem mass spectrometry. J Chromatogr B 2005;823:26–36. 5. Pettitt TR, Dove SK, Lubben A, Calaminus SDJ, Wakelam MJO. The analysis of intact phosphoinositides in biological samples. J Lipid Res 2006;47:1588–1596. 6. Merrill AH, Sullards MC, Allegood JC, Kelly S, Wang E. Sphingolipidomics: Highthroughput, structure-specific and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry.
Boca Raton, FL: CRC Press; 1984:95–240. 42. Body DR. Branch chain fatty acids. In Mangold HK, ed. CRC Handbook of Chromatography: Lipids. Vol. 1. Boca Raton, FL: CRC Press; 1984:241–75. Chapter 2 Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry Trevor R. Pettitt Abstract Lipids are highly dynamic molecules with many different roles ranging from structural to signaling. Alterations in particular lipid levels change cellular behavior. In humans, inappropriate changes may manifest as diseases such as Alzheimer’s, atherosclerosis, diabetes, obesity, and even cancer.
0. 0092. This difference cannot be detected by standard quadrupole and ion-trap instruments. 2 Lipidomic Analysis of Phospholipids and Related Structures 41 References 1. Leslie NR, Downes CP. PTEN function: How normal cells control it and tumour cells lose it. Biochem J, 2004;382:1–11. 2. Han X, Gross RW. Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrom Rev 2005;24:367–412.